Etikèt fliyoresan

 

Koloran fliyoresan yo se zouti enpòtan pou etikèt biomolekil. Dérivés Fluorescein yo ka lajman make sou réactifs ki te itilize nan mikwoskospi fluoresans, flux cytometry, ak deteksyon imunofluorescence. Tipikman, biotin, FITC koloran sa yo ka abitrèman make sou peptides N tèminal oswa C fen. Men, nou rekòmande N End make gen yon pi gwo pousantaj siksè, pi kout tan, ak operasyon pi fasil. Piske peptides yo sòti nan sentèz fen C rive nan fen N, se konsa modifikasyon fen N pral etap final la nan sentèz solid-faz la, elimine nesesite pou etap kouple adisyonèl. Okontrè, si se C End tagging mande pou etap adisyonèl ak pwosesis la pi konplèks.

 

1727320167214663

 

Modifikasyon fluoresans òdinè

Fluoresans modifikasyon Chinwa non N fen N Fini ak linker
Biotin-peptides ki make Biotin- Biotin-Ahx-
fluorescein isothiocyanate FITC- FITC-Ahx-
5-carboxyfluorescein 5-FAM- 5-FAM-Ahx-
dansylfluorescein Dansyl- Dansyl-Ahx-
5-carboxytetramethylrhodamine TMR- (TAMRA-) TMR-Ahx- (TAMRA-Ahx-)

 

Pase Lys Fluorescence modifikasyon nan lyen amine chèn bò

polipeptid N fen sekans polipèptid mwayen polipeptid C fen
Lys(Biotin)- -Lys(Biotin)- -Lys(Biotin)
Lys(FITC)- -Lys(FITC)- -Lys(FITC)
Lys(5-FAM)- -Lys(5-FAM)- -Lys(5-FAM)
Lys(Dansyl)- -Lys(Dansyl)- -Lys(Dansyl)
Lys(TMR)- -Lys(TMR)- -Lys(TMR)
Lys(Dnp)- -Lys(Dnp)- -Lys(Dnp)

 

Longèdonn limyè eksitasyon ak longèdonn limyè emisyon materyèl fliyoresan ke konpayi nou an pwodui souvan

Fliyòfò Egz (nm) Em(nm) Fliyòfò Egz (nm) Em(nm)
Hydroxycoumarin 325 386 R-phycoerythrin (PE) (489) 565 578
Dansyl klori 340 578 Rhodamine Wouj-X 560 580
AMC 345 445 Tamara 565 580
Methoxycoumarin 360 410 Alexa fluor 555 556 573
Alexa fliyò seri 345 442 Alexa fluor 546 556 573
Aminocoumarin 350 445 Rox 575 602
Dabcyl 453 - Alexa fluor 568 578 603
Cy2 490 510 Texas Wouj 589 615
FAM 495 517 Alexa fluor 594 590 617
Alexa fluor 488 494 517 Alexa fliyò 621 639
FITC 495 519 Alexa fluor 633 650 668
Alexa fluor 430 430 545 Cy5 (625) 650 670
5-FAM 492 518 Alexa fluor 660 663 690
Alexa fluor 532 530 555 Cy5.5 675 694
HEX 535 556 TruRed 490; 675 695
5-TAMRA 542 568 Alexa fluor 680 679 702
Cy3 550 570 Cy7 743 767
TRITC 547 572 Cy3.5 581 596